Thermo Fisher Scientific USP28 Polyclonal Antibody
USP28 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC, ELISA 등 다양한 응용에 사용 가능. Human, Mouse, Rat 반응성. Protein A로 정제된 1 mg/mL 액상 항체로, -20°C에서 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human USP28 (amino acids 101–200) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Modification of target proteins by ubiquitin participates in a wide array of biological functions. Proteins destined for degradation or processing via the 26S proteasome are coupled to multiple copies of ubiquitin. Attachment of ubiquitin or ubiquitin-related molecules may also result in changes in subcellular distribution or modification of protein activity.
Deubiquitination is catalyzed by proteases called deubiquitinating enzymes (DUBs), which fall into four distinct families:
- Ubiquitin C-terminal hydrolases
- Ubiquitin-specific processing proteases (USPs)
- OTU-domain ubiquitin-aldehyde-binding proteins
- Jab1/Pad1/MPN-domain-containing metallo-enzymes
Among these, USPs are the most widespread deubiquitinating enzymes across evolution. They release ubiquitin from conjugated proteins and have conserved catalytic domains containing Cys and His boxes, with variable terminal regions that influence substrate recognition, localization, and protein interactions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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