
Thermo Fisher Scientific ZIM2 Monoclonal Antibody (OTI5E12), TrueMAB
인간 ZIM2 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, ICC/IF, Flow cytometry에 적합합니다. 비결합형 형태로 제공되며, 고순도의 친화 크로마토그래피로 정제되었습니다. 연구용으로만 사용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5E12 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–150 and 428–527 of human ZIM2 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
In humans, ZIM2 and PEG3 (GeneID:5178) are distinct genes sharing a set of 5’ exons and a common promoter, both paternally expressed.
Alternative splicing connects shared exons with unique exons specific to each gene — 4 for ZIM2 and 2 for PEG3.
This configuration differs in mouse and cow, where ZIM2 and PEG3 do not share exons, and ZIM2 imprinting is not conserved among mammals.
Alternatively spliced transcripts encoding the same protein have been identified for the human ZIM2 gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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