Thermo Fisher Scientific UBE2E3 Monoclonal Antibody (OTI1B5), TrueMAB

Applications

• Western Blot (WB): 1:2,000

Product Specifications

Target Information

The ubiquitin (Ub) pathway involves three sequential enzymatic steps that facilitate the conjugation of ubiquitin and ubiquitin-like molecules to specific protein substrates. The first step requires ATP-dependent activation of the Ub C-terminus and assembly of multi-Ub chains by the Ub-activating enzyme (E1). The Ub chain is then conjugated to the Ub-conjugating enzyme (E2) to generate an intermediate Ub-E2 complex. The Ub-ligase (E3) catalyzes the transfer of Ub from E2 to the appropriate protein substrate.

UBE2E3 (also designated UBCH9) catalyzes the covalent attachment of ubiquitin to other proteins and is involved in the regulation of transepithelial sodium transport in renal cells. It may also play a role in cell growth arrest. UBE2E3 shuttles between the cytoplasm and nucleus in an IPO11-dependent manner and is ubiquitously expressed at low levels, with high expression in skeletal muscle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.