
Thermo Fisher Scientific Goat anti-Rabbit IgG Fc Cross-Adsorbed Secondary Antibody, HRP
HRP 결합된 염소 항-토끼 IgG Fc 교차흡착 2차 항체로 Western blot, IHC, ELISA에 사용 가능. 높은 특이성과 감도로 신호 증폭에 유리하며, 다양한 검출 시스템과 호환. 친화 크로마토그래피로 정제된 동결건조 형태로 안정적 보관 가능.
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Thermo Fisher Scientific Goat anti-Rabbit IgG Fc Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobin Fc region |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2534795 |
| Target | IgG Fc |
| Cross Adsorption | Against bovine, horse, and human serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
- Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
(Product has been overfilled to ensure complete recovery.) - Centrifuge to remove any particulates.
- Prepare fresh working dilution daily.
- Store lyophilized material at 2–8°C.
- For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
- Based on immunoelectrophoresis, no reactivity is observed to:
- Non-immunoglobulin rabbit serum proteins
- Light chains on all rabbit immunoglobulins
- Rabbit IgG F(ab')₂ fragment
- Serum proteins from bovine, horse, or human
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins.
They are useful in the detection, sorting, or purification of rabbit targets.
Secondary antibodies provide:
- Increased versatility for multiple detection systems (HRP, AP, fluorescence)
- Enhanced sensitivity through signal amplification (multiple secondary antibodies binding to one primary antibody)
These antibodies are typically produced by immunizing the host animal with pooled immunoglobulins from the target species and can be further purified or modified (immunoaffinity chromatography, antibody fragmentation, label conjugation) to generate highly specific reagents.
Available Formats
- [AP]
- [Biotin]
- [FITC]
- [TRITC]
- [Request custom conjugation]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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