Thermo Fisher Scientific Phospho-Claudin 4 (Tyr208) Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:50–1:200

Product Specifications

Product Specific Information

Antibody detects endogenous levels of Claudin 4 only when phosphorylated at Tyr208.

Target Information

Claudin-4 belongs to the 20-member family of claudins, transmembrane cellular adhesion proteins localized at epithelial and endothelial tight junctions. Claudins demonstrate distinct, tissue-specific expression patterns and are essential components of tight junction charge-specific channels that regulate paracellular ion flux. Claudin-4 is a component of tight junction strands and may play a role in internal organ development and function during pre- and postnatal life. Overexpression of Claudin-4 decreases paracellular electrical conductance due to a selective decrease in Na⁺ permeability, with no significant decrease in Cl⁻ permeability. In ovarian carcinoma cells, Claudin-3 and Claudin-4 are upregulated at both mRNA and protein levels. Claudin-4 is also overexpressed in pancreatic cancer tissues and other epithelial cancers. The Claudin-4 gene is deleted in Williams-Beuren syndrome, a neurodevelopmental disorder affecting multiple systems.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.