Thermo Fisher Scientific Tubulin beta Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

10-50 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

30 µg/mL

-

Miscellaneous PubMed (Misc)

-

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat, Xenopus, Zebrafish

Published species

Not Applicable

Host/Isotype

Rabbit / Ig

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic peptide from the c-terminal region of human Tubulin beta chain (TUBB5, TUBB2) conjugated to an immunogenic carrier protein was used as the antigen if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Tubulin,uniProtId:P07437-1,ncbiNodeId:9606,antigenRange:444,antigenLength:444,antigenImageFileName:OST00359G-500UG_beta_Tubulin_P07437-1_Rabbit.svg,antigenImageFileNamePDP:OST00359G-500UG_beta_Tubulin_P07437-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Reconstitute in 500 µL of sterile water. Centrifuge to remove any insoluble material.

The peptide is homologous in many other species including rat, mouse, zebrafish and xenopus.

Specificity of this antibody: Tubulin beta chain.

Target Information

Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.