Thermo Fisher Scientific Tubulin beta Polyclonal Antibody
Rabbit 폴리클로날 항체로 인간, 마우스, 랫, 제브라피시 등에서 Tubulin beta 단백질 검출에 사용. Western blot 및 IHC(P)에서 검증됨. 미세소관 관련 연구 및 로딩 컨트롤용으로 적합. 동결건조 형태로 제공되며 4°C 또는 -20°C에 보관.
- 카탈로그번호
- OST00359G-500UG
- 판매단위
- pk
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 10–50 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 30 µg/mL |
| Miscellaneous PubMed (Misc) | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Xenopus, Zebrafish |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / Ig |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic peptide from the C-terminal region of human Tubulin beta chain (TUBB5, TUBB2) conjugated to an immunogenic carrier protein |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | Not determined |
| Storage Buffer | Whole serum |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for stability. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Reconstitute in 500 µL of sterile water.
- Centrifuge to remove any insoluble material.
- The peptide is homologous in rat, mouse, zebrafish, and xenopus.
- Specificity: Tubulin beta chain.
Target Information
Beta-tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance, and maintenance.
Mutations in the beta-tubulin gene cause congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin is also detected in Sertoli cells and transiently in non-neuronal embryonic tissues.
Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression, inhibiting its incorporation into microtubules.
Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control in protein detection assays such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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