Applied Biosystems™

TaqMan™ Advanced miRNA Human B 96-well Plates, fast

The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays자세히 알아보기

The Applied Biosystems TaqMan Advanced miRNA Human B 96-Well Plates, Fast, provide the convenience of pre-spotted TaqMan Advanced miRNA assays in a 96-well fast plate format. This product contains a set of 4 plates for profiling of up to 376 unique miRNAs, and includes endogenous and exogenous miRNA controls for normalization of data results.

Features of the TaqMan Advanced miRNA Human B 96-Well Plates include:
• Reproducibility—pre-spotted assays are designed to reduce pipetting error and minimize experimental variability
• Sample savings—requires only 2 μL of purified plasma/serum or 1–10 ng of total RNA per sample
• Updated content—assay content updated for mirBase v.21
• Easy to use—just add a mix of diluted miR-Amp and master mix and run the qPCR; no additional pipetting required
• Gold-standard TaqMan qPCR chemistry—TaqMan Advanced miRNA assays draw on Thermo Fisher Scientific`s industry-leading bioinformatics assay design pipeline and verification system to help ensure high specificity and minimal cross-reactivity, even for closely-related miRNA families

Product details:
Number of plates: 4
Number of unique miRNAs (not including exogenous controls): 376
Exogenous controls: cel-miR-39-3p, ath-miR159a
Endogenous control: hsa-miR-16-5p
Compatible with the following Applied Biosystems qPCR instruments: Viia7, StepOnePlus, 7500/7500 Fast, QuantStudio 3, QuantStudio 5, QuantStudio 6, QuantStudio 7 Flex, and QuantStudio 12k Flex
Recommended master mix: TaqMan Fast Advanced Master Mix

Notes
• TaqMan Advanced miRNA plates require the use of the TaqMan Advanced cDNA Synthesis Kit, which uses a universal reverse transcription (RT) chemistry to prepare the cDNA template for use with TaqMan Advanced miRNA assays for detection and quantification of mature human miRNAs in biological samples.
• These plates are not compatible with the first-generation TaqMan MicroRNA cDNA Synthesis Kit, which uses an miRNA-specific reverse transcription chemistry.
• For profiling of miRNAs using miRNA-specific RT, we recommend the TaqMan Array Human MicroRNA B Cards v3.0.

사양

검출 방법Primer-probe

형식96-well plate

GC-Rich PCR PerformanceHigh

PCR 방법2-step RT-qPCR

반응 속도Fast

용도(장비)7500 Fast System, 7900HT Fast System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, ViiA™ 7 System, StepOnePlus™ System

제품라인TaqMan™

제품 유형Advanced miRNA Human 96-Well Plate

수량4 plates

배송 조건Room Temperature

용도(애플리케이션)Microarray Analysis

샘플 종류Liquid Samples (e.g. Serum), Tissue, Cells, FFPE & Fixed Samples, Blood

부피2 μL

Unit SizeEach