Gibco™

NCTC-135 Medium

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated자세히 알아보기

NCTC-135 medium is a chemically defined medium that was originally developed at the National Cancer Institute (Bethesda, MD) and formulated for the culture of mouse Strain L sublines, NCTC clone 929 (L929 cells) in a serum-free environment. Gibco™ NCTC-135 medium has also been used to grow many cells, including HeLa, Ehrlich-Lettre Ascites strain E, NCTC clone 1469, NCTC 4206, mouse C3H, BS-C-1, and hybridoma cell lines.

This NCTC-135 is modified as follows:

With	Without
• L-glutamine	• HEPES
• Phenol Red
• Coenzymes

The complete formulation is available.

Gibco™ NCTC-135 is a modification of NCTC 109 medium that replaced L-cysteine with L-cystine, which reduced toxicity to some cells. NCTC-135 medium is rich in nucleosides contains several unique components to eliminate the need for serum, including coenzymes, glucosamine, reducing agents and additional vitamins.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the labeled intended use may be a violation of local law.

NCTC-135 medium contains no proteins, lipids, or growth factors. NCTC-135 medium was designed for use without serum for L929 cells, but typically requires supplementation of a growth factor cocktail or Fetal Bovine Serum (FBS) for other cells. NCTC-135 medium uses a sodium bicarbonate buffer system (2.2 g / L) and therefore requires a 5-10% CO2 environment to maintain physiological pH. Some cell lines might require gradual adaptation to a serum-free medium.

사양

세포 유형Mammalian Cells

분류Animal Origin-free

농도1 X

Culture TypeMammalian Cell Culture

형태Liquid

제품 유형NCTC-135 Medium

유통 기한12 Months

멸균Sterile-filtered

첨가제 포함Glutamine, Phenol Red, Coenzymes

첨가제 없음No HEPES

제조 품질cGMP-compliant under the ISO 13485 standard

제품라인Gibco™

수량500 mL

배송 조건Room Temperature

Unit SizeEach