Thermo Fisher Scientific CD31/PECAM-1 (Endothelial Cell Marker) Monoclonal Antibody (C31.10)
CD31/PECAM-1 단백질을 인식하는 마우스 단클론 항체로, 인간 및 랫트 시료에 반응합니다. Western blot, IHC, Flow Cytometry 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 액상 형태입니다. 4°C에서 보관하며, 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | C31.10 |
| Immunogen | Recombinant human CD31 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- CF 488 conjugate available
- Custom conjugation available upon request
Target Information
CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor that regulates T cell and B cell signaling through a dual immunoreceptor tyrosine-based inhibitory motif (ITIM). Upon phosphorylation by associated kinases, these motifs provide docking sites for protein-tyrosine phosphatases.
CD31 is ubiquitously expressed within the vascular compartment, primarily at junctions between adjacent endothelial cells. Its N-terminal Ig-like domain mediates homophilic binding, crucial for cell-cell interactions.
This multifunctional molecule plays diverse roles in:
- Integrin-mediated cell adhesion
- Transendothelial migration
- Angiogenesis and apoptosis
- Negative regulation of immunoreceptor signaling
- Autoimmunity and macrophage phagocytosis
- IgE-mediated anaphylaxis and thrombosis
CD31 is one of the key regulatory molecules in the vascular system.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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