Thermo Fisher Scientific DCP1A Monoclonal Antibody (3G4)

Applications and Tested Dilution

Product Specifications

Product Specific Information

Protein Sequence:
STQLSNLGST ETLEEMPSGS QDKSAPSGHK HLTVEELFGT SLPKEQPAVV GLDSEEMERL PGDASQKEPN SFLPFPFEQL GGAPQSETLG VPSAAHHSVQ

Target Information

Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-interacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through an EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF, as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a molecular mass of approximately 70 kDa.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.