Thermo Fisher Scientific DCP1A Monoclonal Antibody (3G4)
DCP1A 단백질을 인식하는 Mouse IgG2a 단클론 항체로 Western blot, ICC/IF, ELISA에 적합합니다. PBS(pH 7.4) 용액 형태로 제공되며, 보존제 없이 -20°C에서 보관합니다. 친화 크로마토그래피로 정제된 고품질 연구용 항체입니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunocytochemistry (ICC/IF) | 10 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3G4 |
| Immunogen | DCP1A (NP_060873, 186–285 a.a.) partial recombinant protein with GST tag (GST tag MW: 26 kDa) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Protein Sequence:
STQLSNLGST ETLEEMPSGS QDKSAPSGHK HLTVEELFGT SLPKEQPAVV GLDSEEMERL PGDASQKEPN SFLPFPFEQL GGAPQSETLG VPSAAHHSVQ
Target Information
Signal transduction of TGF-beta superfamily members is regulated by Smad proteins. Smads influence specific gene transcription by relaying signals from the cell membrane to the nucleus. Smad4 plays an essential role in TGF-beta-induced transcriptional activation wherein phosphorylated receptor-associated Smads associate with Smad4. Furthermore, SMIF (Smad4-interacting protein) and Smad4 complex with TGF-beta and BMP4. An increase in Smad4 concentration increases the translocation of this complex to the nucleus. SMIF and Smad4 interact directly through an EVH1/WH1 domain on SMIF and a proline-rich activation domain on Smad4. Smad4 is essential to nuclear translocation of SMIF, as deletion of the Smad4-interacting domain (located in the N-terminal 100 amino acids) of SMIF eliminates TGF-beta-induced nuclear translocation of SMIF. The human SMIF gene is ubiquitously expressed and encodes a protein with a molecular mass of approximately 70 kDa.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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