Thermo Fisher Scientific Rad50 Polyclonal Antibody
Rabbit polyclonal antibody targeting human Rad50 protein for DNA double-strand break repair studies. Suitable for WB and IHC applications. Unconjugated liquid form, 1 mg/mL concentration, stored at 4°C. Recommended shelf life of 1 year.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:5,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residues 700 and 750 of Human Rad50 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
- Predicted reactivity based on sequence identity: Bovine, Dog, Rabbit, Orangutan, Monkey, Gorilla, Chimpanzee
Target Information
Rad50-13B3 recognizes human Rad50, a 153a protein involved in DNA double-strand break repair.
Rad50 associates with Mre11 and p95 (Nibrin) to form a multiprotein complex essential for the repair process.
Upon DNA damage, Rad50 and Mre11 redistribute and co-localize within the nucleus of damaged cells.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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