Thermo Fisher Scientific EGFR Monoclonal Antibody (H11)
EGFR 단백질을 검출하는 Mouse IgG1 단클론 항체로 Western blot, IHC, ICC/IF, IP 등 다양한 응용에 적합함. Human 및 Mouse 시료 반응성. 단백질 G 정제, PBS 용액 형태로 제공되며 -20°C 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific EGFR Monoclonal Antibody (H11)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.5–1 µg/mL | View 27 publications |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL | View publications |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | View 4 publications |
| Flow Cytometry (Flow) | - | View 3 publications |
| ELISA | - | View 2 publications |
| Immunoprecipitation (IP) | 2 µg/mg lysate | View 3 publications |
| Neutralization (Neu) | - | View 2 publications |
| Gel Shift (GS) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | H11 |
| Immunogen | HC2 20 d2 cells |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1074165 |
Product Specific Information
MA1-12693 detects Epidermal Growth Factor Receptor (EGFR) in human samples.
It has been successfully used in FACS, immunofluorescence, immunoprecipitation, Western blot, and immunohistochemistry (paraffin) procedures.
Staining of formalin-fixed tissues requires digestion with protease XXV at 1 mg/mL in PBS for 5 minutes at 37°C.
Positive controls include A431 cells, placenta, or squamous cell carcinoma.
Suitable for Western blotting of extracellular domain of EGFR.
The immunogen used is HC2 20 d2 cells.
Target Information
EGFR (Epidermal Growth Factor Receptor) is a receptor tyrosine kinase that mediates signals from various growth factors.
Overexpression of EGFR has been associated with multiple cancer types and is a therapeutic target for both biological and small molecule drugs.
The EGFR gene is located on chromosome 7 in humans and encodes a transmembrane receptor belonging to the HER/ERbB family (ERbB2, ERbB3, ERbB4).
Binding of ligands such as EGF and TGF-α induces receptor dimerization and autophosphorylation of tyrosine residues in the cytoplasmic domain.
EGFR overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, and squamous cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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