Thermo Fisher Scientific EGFR Monoclonal Antibody (H11)

Applications

Tested Dilution

Publications

Western Blot (WB)

0.5-1 µg/mL

View 27 publications 27 publications

Immunohistochemistry (IHC)

-

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

2-4 µg/mL

-

Immunocytochemistry (ICC/IF)

5 µg/mL

View 4 publications 4 publications

Flow Cytometry (Flow)

-

View 3 publications 3 publications

ELISA (ELISA)

-

View 2 publications 2 publications

Immunoprecipitation (IP)

2 µg/mg lysate

View 3 publications 3 publications

Neutralization (Neu)

-

View 2 publications 2 publications

Gel Shift (GS)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

H11

Immunogen

HC2 20 d2 cells. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EGFR,uniProtId:P00533-1,ncbiNodeId:9606,antigenRange:1-1210,antigenLength:1210,antigenImageFileName:MA1-12693_EGFR_P00533-1_House_mouse.svg,antigenImageFileNamePDP:MA1-12693_EGFR_P00533-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1074165

Product Specific Information

MA1-12693 detects Epidermal Growth Factor Receptor in human samples.

MA1-12693 has been successfully used in FACS, immunofluorescence, immunoprecipitation, Western blot, and immunohistochemistry (paraffin) procedures. Staining of formalin-fixed tissues requires digestion with protease xxv at 1 mg/mL in PBS for 5 minutes at 37ºC. Positive controls include A431 cells or placenta or squamous cell carcinoma. Suitable for Western blotting of extracellular domain of EGFR.

The MA1-12693 immunogen is HC2 20 d2 cells.

Target Information

EGFR, epidermal growth factor receptor, is a receptor tyrosine kinases that signals in response to various growth factors. Overexpression has been linked to numerous types of cancer and EGFR is the target of both biological and small molecular therapeutics. EGFR is encoded by the EGFR gene located on chromosome 7 in humans. EGFR belongs to the HER/ERbB family of proteins that includes three other receptor tyrosine kinases, ERbB2, ERbB3, ERbB4. EGFR is a transmembrane receptor and binding of its cognate ligands such as EGF (Epidermal Growth Factor) and TGF alpha (Transforming Growth Factor alpha) to the extracellular domain leads to EGFR dimerization followed by autophosphorylation of the tyrosine residues in the cytoplasmic domain. Overexpression is observed in tumors of the head and neck, brain, bladder, stomach, breast, lung, endometrium, cervix, vulva, ovary, esophagus, stomach and in squamous cell carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.