Thermo Fisher Scientific Acetyl-HSP90 (Lys294) Polyclonal Antibody
Acetyl-HSP90 (Lys294) 폴리클로날 항체로 인간, 생쥐, 랫드에서 반응하며 Western blot, IHC, ELISA에 사용 가능. 합성 펩타이드 면역원 기반 친화정제 항체로, 액상 형태이며 -20°C 보관. 스트레스 반응 단백질 HSP90 연구에 적합.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–10 µg/mL |
| ELISA | 1:2,500–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Affinity purified antibody prepared from rabbit serum immunized with a synthetic peptide corresponding to amino acids surrounding K294 of human HSP90 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.96 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form. Western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells treated or untreated with Trichostatin A (an HDAC inhibitor). BLAST analysis suggests cross-reactivity with HSP90 from human, mouse, rat, monkey, chicken, and Drosophila based on 100% homology with the immunizing sequence. Reactivity with HSP90 from other species is unknown.
Target Information
Heat shock proteins (HSP) are expressed in response to various biological stresses, including heat. HSP90 is a 90 kDa protein induced under stress conditions and is abundant under non-stress conditions. It associates with several intracellular proteins, including steroid receptors, actin, tubulin, and kinases. HSP90 is necessary for maintaining the structural integrity of some steroid receptors to allow ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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