Thermo Fisher Scientific Lewis A (Blood Group Antigen) Monoclonal Antibody (7LE)
Lewis A 혈액형 항원에 특이적인 단클론 항체로, 인간 및 마우스 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry 등 다양한 응용에 적합하며, 단백질 A/G로 정제된 액상 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 7LE |
| Immunogen | Mucins isolated from ovarian cyst fluid |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids synthesized by exocrine epithelial cells and circulating in body fluids. These glycosphingolipids are involved in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells, giving rise to their Lewis phenotype.
This gene belongs to the fucosyltransferase family, catalyzing the addition of fucose to precursor polysaccharides in the final step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)- and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for most Lewis antigen-negative phenotypes. Multiple alternatively spliced variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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