Thermo Fisher Scientific GUK1 Monoclonal Antibody (OTI1D7), TrueMAB
Thermo Fisher Scientific의 GUK1 단클론 항체(OTI1D7)는 인간 GUK1 단백질을 표적으로 하는 TrueMAB 형식의 마우스 IgG2b 항체입니다. Western blot, IHC, Flow cytometry 등 다양한 응용에 적합하며, 고순도 친화 크로마토그래피 정제 및 동결건조 형태로 제공됩니다.
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Thermo Fisher Scientific GUK1 Monoclonal Antibody (OTI1D7), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1D7 |
| Immunogen | Full length human recombinant GUK1 protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
- For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Guanylate kinase (GK) catalyzes the ATP-dependent phosphorylation of GMP to GDP, essential for GMP and cGMP recycling. GK is a conserved monomeric protein (~200 amino acids) across species including E. coli, yeast, and vertebrates. Structurally, GK resembles Vaccinia virus protein A57R (SalG2R).
Proteins containing DHR, SH3, and GK-like domains form the MAGUK family, which includes:
- Drosophila tumor suppressor protein dlg1
- Mammalian tight junction protein Zo-1
- Synaptic proteins SAP90/PSD-95, CHAPSYN-110/PSD-93, SAP97/DLG1, SAP102
- Vertebrate erythrocyte membrane protein p55
- C. elegans lin-2
- Rat protein CASK
- Human proteins DLG2 and DLG3
GK has an ATP-binding site (P-loop) in its N-terminal region, which is not conserved in GK-like domains of MAGUKs, though these retain residues for MP binding.
Usage Notice
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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