Applied Biosystems™

TaqMan™ Protein Assays Core Reagents Kit with MasterMix

The TaqMan® Protein Assays Core Reagents Kit with MasterMix contains both the Core Reagents Base Kit and the TaqMan Protein자세히 알아보기

The TaqMan® Protein Assays Core Reagents Kit with MasterMix contains both the Core Reagents Base Kit and the TaqMan Protein Assays Fast Master Mix Kit. The Core Reagents Base Kit contains the reagents required to perform the ligation and PCR amplification steps of TaqMan® protein expression experiments. The Universal PCR Assay contains optimized concentrations of TaqMan® probe and oligonucleotide primers (forward and reverse) that are designed to amplify a region of the protease-treated ligation product for detection on an Applied Biosystems real-time PCR system. The enzyme concentration, ionic strength, and the pH of the TaqMan® Protein Expression Fast Master Mix are optimized for the amplification of ligated oligonucleotide target sequences prepared using TaqMan® Protein Expression Assay reagents. The DNA polymerase included in the master mix is designed for maximum PCR efficiency and specificity when used with the ligation chemistry.

사양

검출 방법Primer-probe

PCR 방법qPCR

중합효소AmpliTaq Fast DNA Polymerase

반응 속도Fast

기술Proximity Ligation Assay

용도(장비)7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, StepOnePlus™, Fast Mode

라벨 또는 염료FAM

패시브 참조 염료ROX (Pre-mixed)

제품라인TaqMan™

제품 유형Protein Assays Core Reagent Kit

수량500 reactions

충분500 Reactions

타겟 특이도Not Target-Specific

핫 스타트No

반응 수500 Reactions

샘플 종류Tissue, Cells

Unit SizeEach