Thermo Fisher Scientific IBP160 Polyclonal Antibody
IBP160 단백질에 대한 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody로, WB, IHC, IP 등 다양한 응용에 적합. 인간 반응성, 항원 친화 크로마토그래피 정제, 액상 형태로 제공되며 4°C에서 보관. 연구용으로만 사용.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:10,000
Immunohistochemistry (IHC)
- Tested Dilution: 1:100–1:500
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:100–1:500
Immunoprecipitation (IP)
- Tested Dilution: 2–5 µg/mg lysate
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 1435 and 1485 of human intron-binding protein of 160 kDa |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Target Information
Involved in pre-mRNA splicing as a component of the spliceosome (PubMed:11991638, PubMed:25599396, PubMed:28502770, PubMed:28076346).
Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP biogenesis (PubMed:16949364).
Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly (PubMed:16949364).
May act by helping the folding of the snoRNA sequence.
Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing (PubMed:16949364).
Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro) (PubMed:25599396).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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