Thermo Fisher Scientific IBP160 Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000–1:10,000

Immunohistochemistry (IHC)

• Tested Dilution: 1:100–1:500

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:100–1:500

Immunoprecipitation (IP)

• Tested Dilution: 2–5 µg/mg lysate

Product Specifications

Product Specific Information

• Recommended shelf life: 1 year from date of receipt
• Application Note: For IHC, epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Target Information

Involved in pre-mRNA splicing as a component of the spliceosome (PubMed:11991638, PubMed:25599396, PubMed:28502770, PubMed:28076346).
Intron-binding spliceosomal protein required to link pre-mRNA splicing and snoRNP biogenesis (PubMed:16949364).
Plays a key role in position-dependent assembly of intron-encoded box C/D small snoRNP, splicing being required for snoRNP assembly (PubMed:16949364).
May act by helping the folding of the snoRNA sequence.
Binds to intron of pre-mRNAs in a sequence-independent manner, contacting the region between snoRNA and the branchpoint of introns (40 nucleotides upstream of the branchpoint) during the late stages of splicing (PubMed:16949364).
Has ATP-dependent RNA helicase activity and can unwind double-stranded RNA molecules with a 3' overhang (in vitro) (PubMed:25599396).
[UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.