Thermo Fisher Scientific DOT1L Monoclonal Antibody (OTI1F6), TrueMAB
DOT1L 단백질을 인식하는 Mouse IgG1 단클론 항체로, Western blot에 적합. 인간 DOT1L(aa 1-434) 재조합 단백질로부터 제작. 동결건조 형태로 제공되며, PBS/trehalose 버퍼에 보관. 연구용으로만 사용 가능.
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Thermo Fisher Scientific DOT1L Monoclonal Antibody (OTI1F6), TrueMAB
Applications
- Western Blot (WB)
Tested Dilution: 1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1F6 |
| Immunogen | Human recombinant protein fragment (aa 1–434 of DOT1L) produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
DOT1L is a histone methyltransferase that methylates Lys-79 of histone H3. Nucleosomes are preferred substrates compared to free histones. The protein binds to DNA.
For Research Use Only. Not for use in diagnostic procedures or resale without express authorization.
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