Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), eFluor 660, eBioscience
마우스 F4/80 항원을 인식하는 BM8 단클론 항체로, eFluor 660 형광체가 결합되어 있습니다. 성숙 대식세포 표지에 적합하며 Flow Cytometry 및 IHC 등 다양한 응용 가능. 651/668 nm 파장에서 동작하며 4°C 암소 보관.
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Thermo Fisher Scientific F4/80 Monoclonal Antibody (BM8), eFluor 660, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 147 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | - | View 4 publications |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 2 publications |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 6 publications |
| Immunocytochemistry (ICC/IF) | - | View 41 publications |
| Flow Cytometry (Flow) | 1 µg/test | View 176 publications |
| Miscellaneous PubMed (Misc) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Published Species | Hamster, Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 660, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BM8 |
| Conjugate | eFluor™ 660 |
| Excitation / Emission Max | 651 / 668 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Wet ice |
| RRID | AB_11150065 |
Available Formats
- Unconjugated
- Alexa Fluor 488
- Alexa Fluor 532
- Alexa Fluor 561
- Alexa Fluor 660
- Alexa Fluor 700
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 661 / 805
- Brilliant Violet 421 / 650 / 711
- eFluor 450 / 506 / 570
- FITC
- Functional Grade
- PE / PE-Cyanine5 / PE-Cyanine7 / PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The BM8 monoclonal antibody reacts with mouse F4/80 antigen, an approximately 160 kDa surface receptor belonging to the EGF-TM7 family. It contains seven EGF-like extracellular domains, seven transmembrane regions, and a cytoplasmic domain homologous to TM7 superfamily members.
F4/80 antigen is expressed by most mature macrophages and is a key marker for this population. It can also be found on peritoneal eosinophils, Langerhans cells, and certain dendritic cell subtypes. Expression begins early in myeloid development and can be upregulated by M-CSF stimulation. Some macrophage subsets, especially in lymphoid tissues, may lack F4/80 expression.
Applications Reported: Flow cytometric analysis.
Applications Tested: Flow cytometric analysis of mouse peritoneal exudate cells. Recommended ≤1 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration for best results.
Fluorochrome Information:
eFluor® 660 replaces Alexa Fluor® 647. Excitation: 633–647 nm; Emission: 668 nm; Laser: Red.
Ensure instrument compatibility for detection.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein, member of the EGF-TM7 family, sharing 68% amino acid identity with human EMR1. Expression varies during macrophage maturation and activation. Found in diverse macrophage populations including Kupffer cells, Langerhans cells, microglia, and macrophages in spleen, thymus, and bone marrow stroma.
Absent in macrophages of T-cell areas of spleen and lymph nodes. Potential role suggested in generation of efferent CD8⁺ regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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