Thermo Scientific™

GeneBLAzer™ GR-UAS-bla HEK 293T Cells

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain자세히 알아보기

GR-UAS-bla HEK 293T cells contain the ligandbinding domain (LBD) of the human Gluocorticoid receptor (GR) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-blaHEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-GR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. GR-UAS-bla HEK 293T cells are functionally validated for Z` and EC50 concentrations of Dexamethasone. In addition, GR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

사양

대체 작용제Tetrac, T4

길항제1-850

어세이 입력Cell-based beta lactamase reporter gene

셀 상태Dividing Cells

검출 방법Fluorescence

종Human

약효를 기대할 수 있는 타겟Nuclear Receptors

셀 수2 x 106 Cells

일차 작용제T3 Hormone

제품라인GeneBLAzer™

제품 유형HEK 293T Cells

수량2 x 106 cells

리포터 유전자BLA (Beta-Lactamase)

타겟 입력NR3C1, GR

Unit SizeEach