Thermo Fisher Scientific EEF1B2 Polyclonal Antibody
EEF1B2 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC, ICC/IF에 사용 가능. Human 및 Mouse에 반응하며, 항원 친화 크로마토그래피로 정제됨. 1 mg/mL 농도의 액상 형태로 제공되며, 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 33–225 of Human EEF1B2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | 0.1M Tris glycine, pH 7, with 10% glycerol |
| Contains | 0.01% thimerosal |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2545950 |
Product Specific Information
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain
- Predicted reactivity: Mouse (96%), Rat (95%), Zebrafish (85%), Xenopus laevis (84%), Pig (98%), Rabbit (99%), Chicken (100%), Rhesus Monkey (100%), Bovine (97%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for the delivery of aminoacyl-tRNAs to the ribosome. The EF-1 complex consists of four subunits: EF-1 α, EF-1 β, EF-1 δ, and EF-1 γ. EF-1 β is a 225 amino acid member of the EF-1 β/EF-1 δ family and contains one GST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange of GDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing of the EF-1 β gene results in three transcript variants differing in the 5′ UTR.
⚠ WARNING: This product can expose you to chemicals including mercury, known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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