Thermo Fisher Scientific EEF1B2 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:10,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

-

Immunocytochemistry (ICC/IF)

1:100-1:1,000

-

Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 33 and 225 of Human EEF1B2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:EEF1B2,uniProtId:P24534-1,ncbiNodeId:9606,antigenRange:33-225,antigenLength:225,antigenImageFileName:PA5-28474_EEF1B2_P24534-1_Rabbit.svg,antigenImageFileNamePDP:PA5-28474_EEF1B2_P24534-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 10% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2545950

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse brain.

Predicted reactivity: Mouse (96%), Rat (95%), Zebrafish (85%), Xenopus laevis (84%), Pig (98%), Rabbit (99%), Chicken (100%), Rhesus Monkey (100%), Bovine (97%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

EF-1 (elongation factor-1) is a multi-protein complex that is responsible forthe delivery of aminoacyl-tRNAs to the ribosome. The EF-1 protein complexis composed of four subunits: EF-1 α, EF-1 β, EF-1 δ and EF-1 γ. EF-1 β is a225 amino acid member of the EF-1 β/EF-1 δ protein family and contains oneGST C-terminal domain. Both EF-1 β and EF-1 δ stimulate the exchange ofGDP bound to EF-1 α to GTP. Phosphorylation of EF-1 β affects the GDP/GTP exchange rate. Alternative splicing events of the gene that encodes EF-1 β result in three transcript variants that differ only in the 5` UTR.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.