Invitrogen™

mMESSAGE mMACHINE™ T3 Transcription Kit

Ambion™ mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kits use Ambion`s patented high-yield transcription technology for the routine synthesis of자세히 알아보기

Ambion™ mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kits use Ambion`s patented high-yield transcription technology for the routine synthesis of 15–35 μg of 7-methyl guanosine capped RNA from 1 μg of template DNA. Each kit includes sufficient reagents for 25 reactions.
• High yields of capped RNA—up to 39 μg of RNA per reaction
• Extra convenience—cap analog-nucleotide mix provided
• Simplified reaction format
• Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 μl reaction during a 2 hour incubation, mMESSAGE mMACHINE™ High Yield Capped RNA Transcription will yield up to 35 μg of capped RNA. This is 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 μg) and proportion of transcripts that are capped (80%). The mMESSAGE mMACHINE™ kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE™ Kits are only optimized for use with the polymerases included in the kit. Using a different polymerase than what is supplied in the kit may result in low yields. Each mMESSAGE mMACHINE™ Kit contains 57 U of cap analog, an outstanding value for the cost.

사양

최종 제품 유형Synthetic mRNA, mRNA

형식Kit

샘플 종류mRNA, Synthetic mRNA

용도(애플리케이션)In Vitro Transcription, synthetic mRNA, mRNA, transfection, LNPs, GENEart, linearized DNA plasmid, delivery, PCR products

반응 수25 Reactions

제품라인Ambion™, mMESSAGE mMACHINE™

제품 유형T3 In Vitro Transcription kit

수량25 Reactions

프로모터T3

배송 조건Dry Ice

수율20-30 μg

시작 물질PCR Products, Linearized DNA plasmid

Unit SizeEach