Thermo Fisher Scientific AP2M1 Monoclonal Antibody (OTI 2F11)
AP2M1 단백질을 인식하는 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC(P), Flow cytometry 등 다양한 응용에 적합. 고순도 친화 크로마토그래피 정제. 인간, 마우스, 랫, 개 종에 반응. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI 2F11 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 97–383 of AP2M1 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.98 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2722869 |
Target Information
The heterotetrameric coat assembly protein complex, also known as the adaptor-related protein complex 2 (AP-2), belongs to the adaptor complexes medium subunits family.
The mu 1 subunit of the AP-2 complex (AP2M1) is required for the activity of a vacuolar ATPase, which is responsible for proton pumping during endosome and lysosome acidification.
AP2M1 associates with the HIV-1 protein Nef, suggesting that Nef may use the AP-2 complex to enhance endocytosis of CD4 and class I MHC.
It may also regulate intracellular trafficking and function of CTLA-4 protein.
At least two isoforms of AP2M1 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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