Gibco™

LIF Recombinant Mouse Protein, embryonic stem cell-qualified

Gibco embryonic stem cell (ESC)–qualified mouse LIF is a recombinant protein that supports the culture and maintenance of mouse pluripotent자세히 알아보기

Gibco embryonic stem cell (ESC)–qualified mouse LIF is a recombinant protein that supports the culture and maintenance of mouse pluripotent stem cells (PSCs) by suppressing spontaneous differentiation. Produced in barley grain to minimize endotoxin levels, each lot is carefully tested for purity, microbial contamination, bioactivity, and ability to support mouse PSC culture, thereby minimizing the risk of contamination and helping ensure robust performance.

With LIF Recombinant Mouse Protein, ESC-Qualified, you can:
• Rely on a recombinant growth factor that is qualified for mouse PSC culture
• Safeguard mouse PSC quality with a low-endotoxin LIF
• Culture with confidence with a rigorously tested reagent

Rely on an ESC-qualified growth factor
LIF bioactivity is typically measured by observing its ability to induce differentiation of murine M1 myeloid leukemia cells. In addition to this standard assay, each lot of ESC-qualified mouse LIF is tested for its ability to maintain Oct4 expression in mouse PSCs, thus helping to ensure robust performance for mouse PSC culture.

Safeguard mouse PSC quality with a low-endotoxin LIF
Unlike bacteria-derived recombinant proteins which may carry over harmful bacterial endotoxins, ESC-qualified mouse LIF is derived from plant tissue. It is verified to contain less than 0.005 ng/μg of endotoxin, more than ten times lower than most bacteria-derived counterparts. This unique, low-endotoxin LIF supports the optimal conditions for safeguarding the health and quality of your mouse PSC culture.

Culture with confidence with a rigorously tested reagent
ESC-qualified mouse LIF is tested for bacteria and mycoplasma to minimize the risk of contamination. It is analyzed by SDS-PAGE to ensure >95% purity. It is also tested for pyrogenic and pro-inflammatory contaminants via monocyte activation test.

사양

용도(애플리케이션)Cell Culture

수량10 μg

유전자 기호LIF

종Mouse

재조합Recombinant

단백질 태그His Tag

분류ES Cell-Qualified

배송 조건Room Temperature

발현 시스템Hordeum vulgare (Barley Grain)

단백질군Cytokines & Receptors

단백질 형태Recombinant

형태Lyophilized

연구 카테고리Stem Cell Research

순도 또는 품질 등급>95% by SDS-PAGE

Unit SizeEach