Thermo Fisher Scientific POLI Polyclonal Antibody
Human POLI 단백질에 특이적인 Rabbit Polyclonal Antibody로 DNA 수선 연구에 활용 가능. IHC(P)에서 1:1,000~1:2,500 희석으로 사용. 항원 친화 크로마토그래피로 정제된 액상 형태. 4°C 단기 보관, -20°C 장기 보관 권장.
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:1,000–1:2,500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Human POLI (Product #RP-107359) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2665164 |
Product Specific Information
Immunogen sequence:
PKDKETNRDFL PSGRIESTRT RESPLDTTNF SKEKDINEFP LCSLPEGVDQ EVFKQLPVDI QEEILSGKSR EKFQGKGSVS CPL
Highest antigen sequence identity to the following orthologs:
- Mouse: 74%
- Rat: 73%
Target Information
Error-prone DNA polymerase specifically involved in DNA repair.
Plays an important role in translesion synthesis, where normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls.
Favors Hoogsteen base-pairing in the active site. Inserts the correct base with high fidelity opposite an adenosine template.
Exhibits low fidelity and efficiency opposite a thymidine template, preferentially inserting guanosine.
May play a role in hypermutation of immunoglobulin genes.
Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but may not have lyase activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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