Thermo Fisher Scientific GFP Recombinant Rabbit Monoclonal Antibody (BLRE04L)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:100–1:500

Immunoprecipitation (IP)

• Tested Dilution: 20 µL/mg of lysate

Product Specifications

Product Specific Information

Immunocytochemistry: Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.

Target Information

The jellyfish Aequorea victoria contains green fluorescent protein (GFP Tag) that emits light in the bioluminescence reaction of the animal. GFP has been widely used as a reporter protein for gene expression in eukaryotic and prokaryotic organisms, and as a protein tag in cell culture and multicellular organisms. As a fusion tag, GFP can be used to localize proteins, study their movement, or research the dynamics of subcellular compartments where these proteins are targeted.

GFP technology has provided significant insights into the physiological activities of living cells. GFP is a 27 kDa monomeric protein that autocatalytically forms a fluorescent pigment. The wild-type protein absorbs blue light (maximally at 395 nm) and emits green light (peak emission 508 nm) without requiring additional proteins, substrates, or cofactors. GFP fluorescence is stable, species-independent, and suitable for a variety of applications, including monitoring gene expression, protein localization, protein–protein interactions (yeast two-hybrid system), and fluorescence energy transfer (FRET) experiments.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.