Invitrogen™

pAd/PL-DEST™ Gateway™ Vector Kit

The pAd⁄PL-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST™ vector for easy recombination-based cloning and adenoviral-based,자세히 알아보기

The pAd⁄PL-DEST™ Gateway™ Vector Kit contains the Gateway™-adapted ViraPower™ adenoviral expression vector, pAd⁄PL-DEST™ vector for easy recombination-based cloning and adenoviral-based, transient expression of a target gene in dividing and non-dividing mammalian cells. The vector allows generation of an adenovirus containing the target gene where expression is driven by a promoter of choice. Alternatively, the vector may also be used to express small RNA molecules from their appropriate promoters.

Advantages
• High efficiency and rapid recombination cloning
• Produces high titer adenoviral stocks
• Efficient delivery of the gene to dividing and non-dividing mammalian cells in vitro or in vivo
• Allows gene of interest to be controlled by a promoter of choice
• Produces replication-incompetent virus for enhanced biosafety of the system
• Amenable for use in high-throughput applications

Key Features
• Gateway™ Technology for efficient and rapid cloning
• Promoterless vector that allows gene of interest to be controlled by a promoter of choice
• Human Ad5 sequences (ΔE3) and Viral Inverted Terminal Repeats (ITRs) for packaging of the expression construct into virions
• Ampicillin selection marker

Kit includes
• pAd⁄PL-DEST™ Vector
• pAd⁄CMV⁄V5-GW⁄lacZ control plasmid

For research use only. Not intended for any therapeutic or diagnostic use.

사양

구성 또는 유도성 시스템Constitutive

배달 유형Adenoviral

프로모터None (Promoterless)

제품 유형Adenovirus Expression Vector

선택 제제(진핵)None

단백질 태그Untagged

클로닝 방법Gateway

수량6 μg

벡터pDEST

제품라인Gateway™, ViraPower™

용도(애플리케이션)Clone your own Promoter, Viral Expression

Unit Size6 µg