Thermo Fisher Scientific Phospho-RAD51 (Tyr315) Polyclonal Antibody
RAD51 단백질의 Tyr315 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능. DNA 복구 관련 연구에 적합하며, 고순도 액상 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 | |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from human RAD51 around the phosphorylation site of Tyr315 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
RAD51 plays a critical role in homologous strand exchange, a key step in DNA repair through homologous recombination. It binds to single- and double-stranded DNA and exhibits DNA-dependent ATPase activity. RAD51 catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. It binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments essential for the homology search and strand exchange. Mutations in the gene can result in breast cancer, mirror movements 2, and Fanconi anemia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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