Thermo Fisher Scientific L1CAM Monoclonal Antibody (OTI2H5), TrueMAB
L1CAM 단백질을 인식하는 마우스 단클론 항체로, Western blot, IHC, ICC, Flow cytometry, IP 등에 사용 가능. 인간 시료에 반응하며, 동결건조 형태로 제공. 신경 발달 관련 연구 및 세포 부착 분자 분석에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2–4 µg/mg |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2H5 |
| Immunogen | Full-length human recombinant L1CAM protein produced in HEK293T cells (CD171, UniProt ID: P32004-1, 1–1257 aa) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For reconstitution, add 100 µL distilled water to achieve approximately 1 mg/mL antibody concentration.
- For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
L1CAM (CD171) is an axonal glycoprotein in the immunoglobulin superfamily. It consists of multiple immunoglobulin-like and fibronectin type III domains linked to a cytoplasmic domain via a transmembrane region. L1CAM is crucial in nervous system development, including neuronal migration and differentiation. Mutations in L1CAM are associated with X-linked neurological syndromes collectively known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus). Alternative splicing of a neuron-specific exon may have functional significance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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