Thermo Fisher Scientific Vimentin Monoclonal Antibody (V9)

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Ready-to-use formulation
• Volume: 150–200 µL

Product Specifications

Product Specific Information

• This product is diluted and in a ready-to-use formulation.
• Recommended positive control tissue: Uterus (not limited to this type).
• Intended for laboratory professional use in detection of vimentin in formalin-fixed, paraffin-embedded tissue stained in manual qualitative IHC testing.
• Reacts with a 58 kDa vimentin protein; no cross-reaction with desmin, keratin, neurofilament, or glial fibrillary acid protein.
• Useful in tumor sub-classification when used in panels with other antibodies.
• Expression of vimentin with anti-keratin aids in distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas.
• Labels mesenchymal cells such as melanocytes, lymphocytes, endothelial cells, and fibroblasts.
• Non-reactivity can assist in identifying tumors lacking vimentin (e.g., hepatoma, seminoma).
• Suitable as a tissue process control reagent.

Usage Notes

• Use with formalin-fixed, paraffin-embedded sections.
• Pretreatment of deparaffinized tissue with heat-induced or enzymatic epitope retrieval is recommended.
• IHC staining involves sequential application of primary antibody, link antibody, enzyme complex, and chromogenic substrate, followed by washing steps.
• Positive tissue control must be run with every staining procedure.
• External positive control materials should be processed identically to patient samples and selected for weak positive staining to ensure reagent sensitivity.
• Internal or external negative controls should be used to confirm absence of non-specific staining.

Target Information

Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin.
It is involved in intracellular transport between the nucleus and plasma membrane and co-expressed with desmin during early development.
Vimentin participates in steroid synthesis regulation by serving as a storage network for cholesterol-containing lipid droplets.
Phosphorylation by protein kinase triggers filament breakdown and cytoskeletal changes that enhance cholesterol transport to mitochondria, increasing steroid synthesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.