Thermo Fisher Scientific PMS2 (Postmeiotic Segregation Increased 2) Recombinant Rabbit Monoclonal Antibody (PMS2, 4373R)
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PMS2 단백질을 인식하는 Thermo Fisher의 재조합 토끼 단일클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. HEK293 세포에서 발현된 IgG 형식의 비결합 항체로, 고순도 Protein A/G 정제. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PMS2, 4373R |
| Immunogen | Synthetic peptide corresponding to residues within aa 1–100 of human PMS2 (UniProt ID: P54278-1) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For immunohistochemistry (PFA-fixed): incubate antibody for 30 min at room temperature.
- Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 min.
Target Information
PMS1 protein homologue 2 (PMS2) is a DNA mismatch repair protein.
Defects in PMS2 are associated with hereditary non-polyposis colorectal cancer (HNPCC).
Human PMS2 is a protein consisting of 862 amino acids.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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