Thermo Fisher Scientific DES-Inducible/Secreted Kit with pCoBlast

Invitrogen™ DES™-Inducible/Secreted Kit with pCoBlast

The DES™-Inducible/Secreted Kit provides the vector pMT/BiP/V5-His for inducible, secreted expression of recombinant proteins and a choice of Blasticidin (pCoBlast) or Hygromycin (pCoHygro) selection.

This vector includes the inducible metallothionein promoter, which is activated by the addition of copper sulfate or cadmium chloride.
The N-terminal signal sequence from the insect BiP gene directs recombinant fusion proteins through the secretory pathway of S2 cells into the culture medium.

주요 특징

• Small vector size (3.6 kb) for improved DNA yield and subcloning efficiency
• C-terminal V5 epitope tag for rapid detection using Invitrogen™ Anti-V5 Antibody
• C-terminal 6xHis tag for easy purification of recombinant fusion proteins using nickel-chelating resin
• Three cloning vectors (A, B, and C) provided, each with a different reading frame relative to the BiP signal sequence

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