Thermo Fisher Scientific Histone H3, phosphorylated (pSer10) Recombinant Rabbit Monoclonal Antibody (PHH3, 471R)
Recombinant rabbit monoclonal antibody recognizing phosphorylated Histone H3 (Ser10). Optimized for IHC (paraffin) applications. Provides high specificity and sensitivity for detecting mitotic cells. Supplied in liquid form at 200 µg/mL, purified by Pr...
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Applications and Tested Dilution
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1–2 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | PHH3, 471R |
| Immunogen | Synthetic peptide corresponding to ARK-pS-TGGKAPRKQLc of Phosphohistone H3 (phospho S10), amino acids 8–21 of the target protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- For IHC (PFA fixed): Incubate antibody for 30 minutes at room temperature.
- For formalin-fixed tissues: Heat tissue sections in 10 mM Tris with 1 mM EDTA (pH 9.0) for 45 minutes at 95°C, then cool at room temperature for 20 minutes before staining.
Target Information
Histone H3 is a DNA-binding protein located in the chromatin of eukaryotic cells. Together with other core histones, it forms nucleosomes, the fundamental units of chromatin structure.
Histones are crucial in transcription regulation, DNA repair, replication, and chromosomal stability.
The N-terminal tail of histone H3 undergoes various post-translational modifications—such as methylation, acetylation, and phosphorylation—that influence chromatin structure and gene expression.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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