Thermo Fisher Scientific SETD7 Monoclonal Antibody (1B3B8)
SETD7 단백질을 표적으로 하는 Thermo Fisher Scientific의 Mouse Monoclonal Antibody (Clone 1B3B8). Western blot, ICC/IF, Flow cytometry 및 ELISA에 적합. 고순도 Protein G 정제, PBS buffer에 0.05% sodium azide 포함. 연구용으로만 사용 가능.
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Thermo Fisher Scientific SETD7 Monoclonal Antibody (1B3B8)
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Human, Non-human primate, Rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1B3B8 |
| Immunogen | Purified recombinant fragment of human SETD7 (AA: 107–366) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_3090520 |
Target Information
Histone methyltransferase that specifically monomethylates Lys-4 of histone H3. H3 Lys-4 methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activation of transcription. Has methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K motif in substrate proteins. Monomethylates Lys-189 of TAF10, increasing its affinity for RNA polymerase II. Monomethylates Lys-372 of p53/TP53, stabilizing and enhancing p53/TP53-mediated transcriptional activation. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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