Thermo Fisher Scientific B-Raf Monoclonal Antibody (1H12)
BRAF 단백질을 인식하는 Mouse monoclonal antibody (clone 1H12). Western blot, IHC, ICC, ELISA에 적합. Human 및 Mouse 시료에서 반응. 정제된 recombinant human BRAF fragment로 면역화됨. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 2 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | View 1 publication |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H12 |
| Immunogen | Purified recombinant fragment of human BRAF expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_10986987 |
Product Specific Information
MA5-15495 targets BRAF in indirect ELISA, IF, IHC, and WB applications and shows reactivity with human and mouse samples.
The immunogen is a purified recombinant fragment of human BRAF expressed in E. coli.
MA5-15495 detects BRAF, which has a predicted molecular weight of approximately 87 kDa.
Target Information
BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It is activated by somatic point mutation in human cancer and is thought to play a role in postsynaptic responses of hippocampal neurons.
This protein regulates the MAP kinase/ERKs signaling pathway, influencing cell division, differentiation, and secretion.
Mutations in BRAF are associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and lung adenocarcinoma.
A pseudogene for BRAF is located on chromosome X.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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