Thermo Fisher Scientific EEF1G Polyclonal Antibody
EEF1G 단백질을 인식하는 Rabbit Polyclonal 항체로, WB, IHC(P), ICC/IF에 사용 가능. Human 시료에 반응하며, 항원 친화 크로마토그래피로 정제됨. 액상 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human EEF1G (Product # RP-97534) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640896 |
Product Specific Information
Immunogen sequence:
CNLITGMFQR LDKLRKNAFA SVILFGTNNS SSISGVWVFR GQELAFPLSP DWQVDYESYT WRKLDPGSEE TQTLVREYFS WEGAFQHVGK A
Highest antigen sequence identity to the following orthologs:
- Mouse: 99%
- Rat: 99%
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for the delivery of aminoacyl-tRNAs to the ribosome. EF-1 gamma (EEF1G), also known as GIG35, is a 437 amino acid subunit of the EF-1 complex. It is expressed in various tissues including stomach, pancreas, brain, lung, kidney, intestine, liver, and spleen.
EEF1G contains an N-terminal Glutathione transferase domain that may anchor the complex to cellular components and play a role in assembling multiprotein complexes containing aminoacyl-tRNA synthetases. Elevated expression of EF-1 gamma has been associated with pancreatic cancer, suggesting a potential role in oncogenic transformation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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