Thermo Fisher Scientific CD16-2 Recombinant Rabbit Monoclonal Antibody (012)
Thermo Fisher Scientific의 CD16-2 재조합 토끼 단클론 항체(클론 012)는 Mouse CD16-2/FCGR4 특이성을 가지며 높은 재현성과 감도를 제공합니다. ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 적합하며, 보존제가 없는 액상 형태로 제공됩니다. 장기 보관은 -20°C에서 권장됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 1:50–1:1,000 |
| Flow Cytometry (Flow) | 1:25–1:100 |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 012 |
| Immunogen | Recombinant Mouse FCGR4 protein (Met1–Gln203) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.18 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785533 |
Additional Formats
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide (final concentration 0.05%–0.1%) to avoid contamination.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. These systems clone antibody DNA sequences from immunoreactive rabbits, followed by screening of individual clones to select optimal candidates for production.
Advantages of recombinant rabbit monoclonal antibodies:
- Better specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity due to larger rabbit immune repertoire
This antibody has specificity for Mouse CD16-2/FCGR4.
Target Information
FcgRIV (CD16-2) is an intermediate-affinity activating receptor for IgG2a and IgG2b. CD16-2 is the mouse homolog of human FcgRIIIA and acts as a low-affinity IgE receptor for all allotypes. Ligation of FcgRIV by antigen-IgE immune complexes promotes macrophage-mediated phagocytosis and contributes to lung inflammation.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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