Thermo Fisher Scientific PHC1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PHC1. Recombinant protein control fragment (Product #RP-89502). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PHC1,uniProtId:P78364-1,ncbiNodeId:9606,antigenRange:142-225,antigenLength:1004,antigenImageFileName:PA5-52370_PHC1_P78364-1_Rabbit.svg,antigenImageFileNamePDP:PA5-52370_PHC1_P78364-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2645474

Product Specific Information

Immunogen sequence: LNQSQAQMYL RPQLGNLLQV NRTLGRNVPL ASQLILMPNG AVAAVQQEVP SAQSPGVHAD ADQVQNLAVR NQQASAQGPQ MQGS

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 96%.

Target Information

Polycomb group (PcG) proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes throughout development. PHC1 (polyhomeotic homolog 1), also known as EDR1, HPH1 or RAE28, is a 1,004 amino acid nuclear protein that is a component of the PcG multiprotein PRC1 complex. Specifically, the PcG PRC1 complex modifies histones, remodels chromatin and mediates monoubiquination of Histone H2A. Other constituent proteins involved in the PcG PRC1 complex are Mel-18, Bmi-1, M33, MPc2, MPc3, RING1, Ring1b, as well as several others. Existing as a homodimer, PHC1 contains one FCS-type zinc finger and a SAM (sterile alpha motif) domain. PHC1 is encoded by a gene located on human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.