Thermo Fisher Scientific HEC1 Polyclonal Antibody
HEC1 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인간·마우스·랫트 시료에 반응. Western blot, IHC, ICC/IF에 적합. 고순도 Affinity chromatography 정제, PBS/glycerol buffer로 안정적. 연구용으로 세포 분열 관련 스핀들 체크포인트 연구에 활용.
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Thermo Fisher Scientific HEC1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NDC80 (Accession O14777), corresponding to amino acid residues Y611–E642 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2852156 |
Product Specific Information
Antibody detects endogenous levels of total NDC80.
Target Information
Hec1 is one of several proteins involved in spindle checkpoint signaling. This surveillance mechanism assures correct segregation of chromosomes during cell division by detecting unaligned chromosomes and causing prometaphase arrest until the proper bipolar attachment of chromosomes is achieved.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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