Thermo Fisher Scientific Phospho-AMPK alpha-1,2 (Thr183, Thr172) Polyclonal Antibody
AMPK α1/α2의 Thr183/Thr172 인산화를 검출하는 Rabbit Polyclonal 항체로, WB, IHC, ELISA, IP에 적합. Human, Mouse, Rat 반응성. 액상 형태로 0.79 mg/mL 농도, -20°C 보관. 에너지 대사 연구에 유용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A phospho-specific peptide corresponding to residues surrounding T172 of human AMPK alpha |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.79 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2855562 |
Target Information
The protein encoded by this gene is a catalytic subunit of AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit and non-catalytic beta and gamma subunits. It acts as an energy-sensing enzyme that monitors cellular energy status. Upon metabolic stress, AMPK is activated and phosphorylates key enzymes such as acetyl-CoA carboxylase (ACC) and HMG-CoA reductase (HMGCR), which regulate fatty acid and cholesterol biosynthesis. Studies in mice suggest that this catalytic subunit is important for insulin sensitivity and myocardial energy homeostasis during ischemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
배송 정보
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