Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
HRP 결합 F(ab')2 염소 항-토끼 IgG 2차 항체로, WB, IHC, ELISA에 적합합니다. 교차흡착 처리로 비특이적 결합 최소화하며, 높은 감도와 신뢰성 있는 신호 검출을 제공합니다. 리오필라이즈 형태로 안정적 장기 보관 가능.
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Thermo Fisher Scientific F(ab`)2-Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rabbit |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2536005 |
| Target | IgG |
| Cross Adsorption | Against bovine, human and mouse IgG and serum proteins |
| Antibody Form | F(ab`)2 Fragment |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve.
(Product has been overfilled to ensure complete recovery.)
Centrifuge to remove any particulates. Prepare fresh working dilution daily.
Store lyophilized material at 2–8°C. For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on Immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin rabbit serum proteins, serum proteins from bovine, human, or mouse, IgG from human or mouse.
Target Information
Anti-Rabbit secondary antibodies are affinity-purified antibodies with well-characterized specificity for rabbit immunoglobulins and are useful in the detection, sorting, or purification of its specified target.
Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g., HRP, AP, fluorescence).
They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody.
Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
Additional Formats Available
- Alexa Fluor Series (350–790, Plus series)
- DyLight Series (350–800)
- FITC, PE, APC, Biotin, Rhodamine, Texas Red, TRITC, Pacific Blue, Pacific Orange
- Enzyme conjugates (AP, HRP)
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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