Thermo Fisher Scientific Vimentin Polyclonal Antibody
Thermo Fisher Scientific의 Vimentin Polyclonal Antibody는 사람, 생쥐, 랫트 시료에 반응하며 WB, IHC, ICC, ELISA 등 다양한 응용에 적합합니다. 고순도 친화 크로마토그래피로 정제된 액상 항체로, 세포골격 연구 및 단백질 운반 메커니즘 분석에 유용합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing amino acids 1–101 of human Vimentin (NP_0033712) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.67 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2901169 |
Product Specific Information
- Positive Samples: Jurkat, HeLa
- Immunogen sequence:
MSTRSVSSSS YRRMFGGPGT ASRPSSSRSY VTTSTRTYSL GSALRPSTSR SLYASSPGGV YATRSSAVRL RSSVPGVRLL QDSVDFSLAD AINTEFKNTR T
Target Information
Vimentin is a developmentally regulated intermediate filament protein (IFP) found in cells of mesenchymal origin. It is involved in intracellular transport of proteins between the nucleus and plasma membrane. Vimentin, along with desmin, is expressed during early cellular development and later replaced by tissue-specific IFPs. It plays a role in steroid synthesis by acting as a storage network for cholesterol-containing lipid droplets. Phosphorylation of vimentin induces filament breakdown and cytoskeletal reorganization, facilitating cholesterol transport to mitochondria and enhancing steroidogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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