
Thermo Fisher Scientific CRYAB Monoclonal Antibody (OTI6D11), TrueMAB
CRYAB 단백질을 인식하는 Mouse IgG1 단일클론 항체로, WB, IHC, ICC, Flow, IP 등 다양한 실험에 사용 가능. Human 및 Rat 반응성. Lyophilized 형태로 제공되며, PBS buffer에 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
| Flow Cytometry (Flow) | 1:100 |
| Immunoprecipitation (IP) | 2 µg/500 µL |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI6D11 |
| Immunogen | Full length human recombinant CRYAB protein produced in HEK293T cells |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Lens proteins consist primarily of crystallins (about 95%), which also occur in vertebrate skeletal muscle tissue.
Crystallins maintain the proper refractive index of the lens and include three major classes: alpha, beta, and gamma.
Alpha-crystallin, the largest class, consists of alpha-A and alpha-B gene products. Alpha-B crystallin functions as a molecular chaperone, preventing protein aggregation that may lead to cataract formation.
Phosphorylated states of alpha-crystallin respond to cellular stress and may play a role in protein maintenance.
Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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