Invitrogen™

LIVE/DEAD™ _Funga_Light™ Yeast Viability Kit, for flow cytometry

The LIVE/DEAD™ FungaLight™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes자세히 알아보기

The LIVE/DEAD™ _Funga_Light™ Yeast Viability Kit enables researchers to easily, reliably and quantitatively distinguish live and dead yeast in minutes by flow cytometry. The kit contains solutions of the SYTO 9 green-fluorescent nucleic acid stain and the red-fluorescent nucleic acid stain, propidium iodide. These stains differ both in their spectral characteristics and in their ability to penetrate healthy yeast cells. When used alone, the SYTO 9 stain generally labels all yeast in a population - those with intact membranes and those with damaged membranes. In contrast, propidium iodide penetrates only yeast with damaged membranes, causing a reduction in the SYTO 9 stain fluorescence by fluorescence resonance energy transfer (FRET) when both dyes are present. As a result, yeast with intact membranes stain fluorescent green, whereas yeast with damaged membranes stain fluorescent red.

Any physiological buffer between pH 7.0–8.0, including PBS, can be used to dilute the SYTO dyes for the staining solution.

사양

세포 투과성Impermeant, Permeant

색상Green, Red

설명LIVE/DEAD™ FungaLight™ Yeast Viability Kit, for flow cytometry

검출 방법Fluorescence

용도(애플리케이션)Viability Assay

용도 (장비)Flow Cytometer

제품 유형Yeast Viability Kit

염료 유형SYTO™ 9, propidium iodide

방출498/617 nm

여기 파장 범위485/535 nm

형태Solution

형식Tube(s)

제품라인FungaLight™, LIVE/DEAD™

수량200 Assays kit

배송 조건Room Temperature

Unit SizeEach