Thermo Fisher Scientific Dinitrophenyl-KLH Polyclonal Antibody, Alexa Fluor 488

Applications and Tested Dilution

Product Specifications

Product Specific Information

The anti-DNP-KLH antibody is supplied in 0.1 M sodium phosphate, 0.1 M sodium chloride, pH 7.5, containing 5 mM sodium azide. The degree of labeling is indicated on the product label. This antibody was raised in rabbit against DNP conjugated to keyhole limpet hemocyanin. The IgG fraction, which contains both anti-DNP and anti-KLH IgGs, was isolated from total serum and determined to be >95% pure by SDS-PAGE.

It is recommended to briefly centrifuge protein conjugate solutions before use and use only the supernatant to avoid aggregates that may cause nonspecific background staining. Appropriate antibody dilution should be determined empirically for each application.

Peak absorption and fluorescence emission wavelengths for Alexa Fluor™ 488 anti-DNP-KLH conjugate are 494 nm and 520 nm, respectively.

Because of their high affinity for the dinitrophenyl hapten (DNP), Invitrogen’s anti-DNP-KLH antibodies are sensitive probes for detecting DNP-labeled molecules. The fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes.

Anti-DNP antibody binds well to DAMP, an acidotropic reagent that penetrates cell membranes, enabling immunochemical localization of intracellular compartments with low pH. Additionally, anti-DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing isolation of cell-surface molecules of human melanoma cells labeled with trinitrobenzene sulfonic acid (TNBS).

Target Information

DNP-KLH antibodies are used for detecting DNP-labeled molecules. Fluorescent and biotinylated anti-DNP-KLH conjugates can serve as second-step reagents for signal amplification in protein and nucleic acid detection systems, including in situ hybridization studies employing DNP-modified DNA probes. Because anti-DNP antibody binds well to DAMP (D-1552), an acidotropic reagent that can penetrate cell membranes, anti-DNP-KLH antibodies can also be used with DAMP for immunochemical localization of intracellular compartments with low pH. DNP-KLH antibodies may recognize compounds with a trinitrophenyl group, allowing the isolation of cell-surface molecules of human melanoma cells that have been labeled with trinitrobenzene sulfonic acid (TNBS).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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