Takara Vaccinia Capping Enzyme

7-methylguanylate cap structures (Cap-0) are essential for efficient translation of in vitro-transcribed RNA in eukaryotic expression systems. Vaccinia Capping Enzyme provides all the components necessary to add Cap-0 structures to the 5′ ends of uncapped RNA. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor.

7-methylguanylate cap structures (Cap-0) are essential for efficient translation of in vitro-transcribed RNA in eukaryotic expression systems. Vaccinia Capping Enzyme provides all the components necessary to add Cap-0 structures to the 5′ ends of uncapped RNA. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor.

The use of the Vaccinia Capping Enzyme in the same reaction with mRNA Cap 2′-O-Methyltransferase (Cat. # 2470) generates Cap-1 RNA. Cap-1 RNA has a methyl group at the 2`-O position of the first nucleotide adjacent to the cap structure at the 5´ ends of the RNA. This capping modification reduces the innate immune response to in vitro transcribed RNA when it is used in vivo.

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