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카탈로그 번호 | 설명 | 상태 | 단위 | 가격 | 가격(VAT포함) | 수량 / 장바구니 / 찜 |
2460A | Takara 2460A Vaccinia Capping Enzyme, 500 U pk | 재고문의 | pk | 276,000원 | 303,600원 | |
2460B | Takara 2460B Vaccinia Capping Enzyme, 2,000 U pk | 재고문의 | pk | 0원 | 0원 |
7-methylguanylate cap structures (Cap-0) are essential for efficient translation of in vitro-transcribed RNA in eukaryotic expression systems. Vaccinia Capping Enzyme provides all the components necessary to add Cap-0 structures to the 5′ ends of uncapped RNA. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor.
7-methylguanylate cap structures (Cap-0) are essential for efficient translation of in vitro-transcribed RNA in eukaryotic expression systems. Vaccinia Capping Enzyme provides all the components necessary to add Cap-0 structures to the 5′ ends of uncapped RNA. RNA transcripts can be capped in less than one hour in the presence of the capping enzyme, reaction buffer, GTP, and a methyl donor.
The use of the Vaccinia Capping Enzyme in the same reaction with mRNA Cap 2′-O-Methyltransferase (Cat. # 2470) generates Cap-1 RNA. Cap-1 RNA has a methyl group at the 2`-O position of the first nucleotide adjacent to the cap structure at the 5´ ends of the RNA. This capping modification reduces the innate immune response to in vitro transcribed RNA when it is used in vivo.
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