Thermo Fisher Scientific Nestin Polyclonal Antibody
Nestin 단백질을 인식하는 고순도 Rabbit Polyclonal 항체로, 인간·마우스·랫트 시료에 반응합니다. Western blot, IHC, ICC/IF 등 다양한 응용에 적합하며, 줄기세포 및 신경전구세포 마커 연구에 활용됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:50–1:100 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide of human NES |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2803846 |
Product Specific Information
Purity is >95% by SDS-PAGE.
Target Information
Nestin is a Class VI intermediate filament abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells.
It is widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells.
Nestin serves as a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors.
Highly specific antibodies to human Nestin can aid in characterizing progenitor cells differentiating into distinct lineages and in enhancing the therapeutic potential of human neural stem/progenitor cells for CNS disease or injury treatment.
Nestin expression has been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker of proliferating endothelial cells in colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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