Thermo Fisher Scientific EEF2 Recombinant Rabbit Monoclonal Antibody (20H2L1)
EEF2 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot과 Immunocytochemistry에 적합합니다. 높은 특이성과 재현성을 제공하며, 로트 간 일관성이 우수합니다. 단백질 A로 정제된 액상 형태로 제공되며, 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | Expi293 |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 20H2L1 |
| Immunogen | Protein corresponding to human EEF2 (aa241–aa340) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Protein A |
| Storage buffer | PBS, pH 7.4 |
| Contains | 0.09% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2716895 |
Product Specific Information
This antibody is predicted to react with Monkey, Pig, Bovine, and Sheep.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The specific antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal clones for production.
Advantages include:
- Superior specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broader immunoreactivity due to the rabbit immune repertoire
Target Information
EEF2 catalyzes the GTP-dependent ribosomal translocation step during translation elongation. This process shifts the ribosome from the pre-translocational (PRE) to the post-translocational (POST) state as A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively, coordinating tRNA, mRNA, and ribosomal conformational changes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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