Thermo Fisher Scientific TCL1 Monoclonal Antibody (ZM92), MonoMab

Applications

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: Ready-to-use (150–200 µL)

Product Specifications

Product Specific Information

• This antibody is diluted and ready-to-use.
• Recommended positive control tissue: Lymph Node (other tissues may also be suitable).
• Intended for professional laboratory use in detecting TCL1 protein in formalin-fixed, paraffin-embedded tissue using manual qualitative IHC.
• Used after primary tumor diagnosis by conventional histopathology.
• TCL1A (p14TCL1) is involved in T-cell prolymphocytic leukemia (TPLL), which is associated with chromosomal rearrangements between TCRA locus (14q11) and TCL1A gene (14q32.13).
• TCL1 is overexpressed in various lymphomas including Burkitt, AIDS-related non-Hodgkin, lymphoblastic, chronic lymphocytic, mantle cell, follicular, and diffuse large B-cell lymphoma.
• Pretreatment by heat-induced or enzymatic epitope retrieval is recommended.
• IHC staining visualizes antigens via sequential application of primary antibody, secondary antibody, enzyme complex, and chromogenic substrate with washing steps.
• Results are interpreted microscopically to aid differential diagnosis.

Quality Control Guidance

• A positive tissue control must be run with every staining procedure.
• Control tissues should include both positive and negative staining areas.
• External positive controls should be fresh specimens processed identically to patient samples.
• Weakly positive tissues are preferred for detecting subtle reagent degradation.
• Negative controls (internal or external) verify absence of non-specific staining and ensure validity of results.

Target Information

• Overexpression of TCL1 gene is implicated in mature T-cell leukemia due to chromosomal rearrangements near TCR regulatory elements (Virgilio et al., 1998).
• In normal T cells, TCL1 is expressed in CD4-/CD8- cells but not in later differentiation stages.
• TCL1 acts as a coactivator of the cell survival kinase AKT (Laine et al., 2000).

For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.