Thermo Fisher Scientific Chicken anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, HRP
Thermo Fisher Scientific의 Chicken anti-Mouse IgG (H+L) HRP 결합 2차 항체는 Western blot, IHC, ELISA에 적합합니다. 닭 유래 다클론 항체로, 교차 흡착 처리되어 비특이적 반응을 최소화합니다. 동결건조 형태로 제공되며, HRP 결합으로 높은 감도 검출이 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Chicken / IgY |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | HRP |
| Form | Lyophilized |
| Concentration | 2.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 1% BSA |
| Contains | 0.1% ProClin 150 |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2534655 |
| Target | IgG |
| Cross Adsorption | Against bovine, horse and human serum proteins |
| Antibody Form | Whole Antibody |
Product Specific Information
Rehydrate with 0.55 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily. Store lyophilized material at 2–8°C.
For long-term storage after reconstitution, dilute with 50% glycerol and store at -20°C as a liquid.
Based on immunoelectrophoresis, no reactivity is observed to: non-immunoglobulin mouse serum immunoglobulins, IgG/serum from human or rabbit.
Target Information
Mouse IgG is an immunoglobulin G antibody produced by B cells in response to an antigen. It is the most abundant immunoglobulin in serum and plays a crucial role in the adaptive immune response.
It is composed of two heavy chains and two light chains and is involved in opsonization, complement activation, and antibody-dependent cell-mediated cytotoxicity.
Mouse IgG is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry, as well as in therapeutic applications such as immunotherapy and vaccine development.
Additional Formats Available
- Alexa Fluor 488 conjugate
- Alexa Fluor 594 conjugate
- Alexa Fluor 647 conjugate
- Biotin conjugate
- TRITC conjugate
- Custom conjugation available upon request
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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